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The results from the present study indicated that RPA assay can be used easily in routine indexing of citrus planting material. To the best of our knowledge, this is the first report on development of a rapid and simplified isothermal detection assay for CYMV and can be utilized as an effective technique in quarantine and budwood certification process.
We demonstrated its clinical utility in large sample volumes from 46 clinical specimens including environmental swabs, saliva, and blood plasma. The SLIM system showed higher sensitivity with these samples and could detect pathogens that were below the threshold of detection with other methods.
The aim of the present study was to compare the diagnostic ability of nested-multiplex PCR and Recombinase polymerase amplification for differential detection of E. histolytica and E. dispar. The results of this study showed good agreement between the two tests. This suggests that RPA can be used as clinical and epidemiological tool for diagnosis of amoebiasis in resource-limited setups and
The dengue RT-RPA assay can be successfully performed by simply following the provided written instructions. Deviations from the written protocols did not adversely affect the outcome of the assay. These suggest that the RT-RPA assay is indeed a simple, robust and efficient laboratory method for detection of dengue virus.
RPA tests displayed 100% specificity and sensitivity. The hands-off turnaround time at 42°C ranged from 5 to 6 min for 102 genomic copies. The analytical sensitivity of each RPA was ∼10 molecules per reaction. Besides, BA_5345 and adk RPA assays were assessed in field conditions on a series of surface swabs (n=13 with 11 swabs contaminated with B. thuringiensis spores)
The detection limit of Pseudomonas aeruginosa in real-time PCR and PCR was 1×10 7 CFU/mL, and the detection limit of Pseudomonas aeruginosa in RPA was 1×10 2 CFU/mL.RPA. In fluorescence quantitative PCR, the higher the concentration of Pseudomonas aeruginosa, the shorter the threshold time and the fewer the number of cycles, i.e. the shorter the time for detecting the positive
The assay had a detection limit of 134 copies per μL of plasmid containing the NP gene of Ebolavirus Mayinga, and cultured Ebolavirus and was highly specific for the Zaire ebolavirus species, including the Guinea strain responsible for the 2014/2015 outbreak.
The analytical sensitivity in the multiplex assay amplifying specific regions of S. pneumoniae and L. pneumophila simultaneously was 10 CFUs of genomic DNA per reaction. In cross detection studies with closely related strains and other bacterial agents the specificity of the RPA was confirmed.
A recombinase polymerase amplification assay for the diagnosis of atypical pneumonia Read More
The assay performance was further evaluated by testing 60 canine fecal samples, and CPV-2 DNA was detected in 46 samples (76.7%, 46/60) by LFS RPA, which was the same result as that of the real-time PCR assay and higher than that of the SNAP method (48.3%, 29/60).
A novel PKD-RPA assay for the detection of T. bryosalmonae was developed. The assay offers considerable advantages including speed, sensitivity, specificity and visual detection. Applying the PKD-RPA assay combined with an LFD enhances the surveillance and early detection of T. bryosalmonae in salmonids.
