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Using this enrichment platform, the detection limit was found to be 20 times more sensitive in 10 ml urine with Salmonella and 10 times more sensitive in 10 ml urine with Brucella than that of real-time PCR without the enrichment process.
This technique showed a high specificity and sensitivity to amplify the genomic DNA of F. hirta and allowed for rapid amplification (within 15 min) of the ITS region under a constant and mild temperature range of 37–42 °C without using thermocyclers.
All results were compared with real-time PCR assays directed against the same rickettsial genes. The RPA assays are easy to handle and produced quicker results in comparison to real-time PCR.
The assay performance on acute-phase high fever clinical samples collected from cattle with no vaccine against IBRV, which were suspected to be infected with IBRV, was validated by detecting 24 fecal, 36 blood, 38 nasal swab and 8 tissue specimens, and compared with SYBR Green I based real-time PCR.
This method is rapid and efficient, and has good specificity and sensitivity, which is suitable for the rapid identification of porcine ingredient in meat from the market.
•A fast and label-free Mycobacterium tuberculosis diagnostic platform is developed. •The detection relies on asymmetric isothermal amplification and microring sensors. •The platform could detect a single cell of Mycobacterium tuberculosisH37Rv. •Both single and multiplex detection of TB biomarkers in clinical sputa are shown.
This is the first report of RPA-based assays for the detection of “Ca. P. mali”. The assays are suitable for high-throughput screening of plant material and point-of-care diagnostic and can be potentially combined with a simplified DNA extraction procedure.
•A rapid virus diagnostic system for human adenovirus. •Combination system of a microfluidic sample processing and a bio-optical sensor. •Use of a non-chaotropic reagent with a disposable thin-film platform in a single microchannel for sample processing. •Validated the clinical sensitivity of the virus diagnostic system in 13 clinical specimens. •This virus diagnostic system offers a rapid (
Rapid virus diagnostic system using bio-optical sensor and microfluidic sample processing Read More
Here, novel assays, i.e. HighSpeed RT-qPCR and isothermal recombinase polymerase amplification (RPA) were designed and tested. Furthermore, three magnetic bead-based rapid extraction methods for manual or automated extraction were validated and combined with the new downstream assays.
Development of molecular confirmation tools for swift and easy rabies diagnostics Read More
•LAMP and RPA methods were developed for the detection of L. monocytogenes. •Performance of the new methods was compared against two reference real-time PCR methods. •A very low limit of detection, with high confidence, was obtained for both methodologies. •Both methodologies proved reliable alternatives to conventional culture methods.
