PublicationsPost Type
The ISAD-KRAS assay provides a rapid, highly sensitive, and label-free method for KRAS mutation testing, and can serve as a robust and near patient testing approach for the rapid detection of patients most likely to respond to anti-EGFR drugs.
Tests of recombinant plasmid-spiked serum samples revealed that RPA and real-time PCR had the same diagnostic rate. The RPA assay, which is simple, cost-effective, and fast, is a promising alternative to real-time PCR for ASFV detection.
In this work, it was shown for the first time that the combination of a propidium monoazide (PMA) treatment with haRPA, the so-called viability haRPA, is able to identify viable Legionella on DNA microarrays.
This method is rapid and efficient, and has good specificity and sensitivity, which is suitable for the rapid identification of porcine ingredient in meat from the market.
In this study, we introduced a rapid and visual detection method of MTBC using recombinase polymerase amplification (RPA) combined with lateral flow (LF) strips. The LF-RPA assay, read results with naked eyes, could detect as few as 5 genome copies of M. tuberculosis H37Rv (ATCC 27294) per reaction and had no cross-reactions with other control bacteria even using excessive amount of template DNA.
•A fast and label-free Mycobacterium tuberculosis diagnostic platform is developed. •The detection relies on asymmetric isothermal amplification and microring sensors. •The platform could detect a single cell of Mycobacterium tuberculosisH37Rv. •Both single and multiplex detection of TB biomarkers in clinical sputa are shown.
•A rapid virus diagnostic system for human adenovirus. •Combination system of a microfluidic sample processing and a bio-optical sensor. •Use of a non-chaotropic reagent with a disposable thin-film platform in a single microchannel for sample processing. •Validated the clinical sensitivity of the virus diagnostic system in 13 clinical specimens. •This virus diagnostic system offers a rapid (
Rapid virus diagnostic system using bio-optical sensor and microfluidic sample processing Read More
Here, novel assays, i.e. HighSpeed RT-qPCR and isothermal recombinase polymerase amplification (RPA) were designed and tested. Furthermore, three magnetic bead-based rapid extraction methods for manual or automated extraction were validated and combined with the new downstream assays.
Development of molecular confirmation tools for swift and easy rabies diagnostics Read More
•A real-time RPA assay based on exo probe was developed for the rapid detection of PCV3. •The assay showed high analytical sensitivity and specificity for PCV3 detection. •Clinical samples collected from 2014 to July 2017 were used for further assay optimization. •Real-time RPA was shown to be a simple, rapid and reliable method for PCV3 detection.
Recombinase polymerase amplification assay for rapid detection of porcine circovirus 3 Read More
The presented methodology possesses several advantages over existing methods, i.e., it is fast, achieving the detection of DNA produced after only 5 min of amplification, and simple, since it does not require any post-amplification DNA extraction step.
