Publications Archive - Page 20 of 35

Development of a rapid diagnostic assay for the detection of tomato chlorotic dwarf viroid based on isothermal reverse-transcription-recombinase polymerase amplification.

A molecular diagnostic assay utilizing reverse transcription-recombinase polymerase amplification (RT-RPA) at an isothermal constant temperature of 39 °C and target-specific primers and probe were developed for the rapid, sensitive, and specific detection of tomato chlorotic dwarf viroid (TCDVd) in infected leaf and seed tissues.

Development of a rapid diagnostic assay for the detection of tomato chlorotic dwarf viroid based on isothermal reverse-transcription-recombinase polymerase amplification. Read More

A simple, rapid, low-cost technique for naked-eye detection of urine-isolated TMPRSS2:ERG gene fusion RNA.

By itineris_a2nfh3osnde8e4

We describe herein a simple, rapid and inexpensive assay which combines robust isothermal amplification technique with a novel visualization method for evaluating urinary TMPRSS2:ERG status at less than USD 5 and with minimal equipment.

A simple, rapid, low-cost technique for naked-eye detection of urine-isolated TMPRSS2:ERG gene fusion RNA. Read More

Enhanced solid-phase recombinase polymerase amplification and electrochemical detection

By itineris_a2nfh3osnde8e4

Here, we elucidate the optimal surface chemistry for rapid and efficient solid-phase RPA, which was fine-tuned in order to obtain a maximum signal-to-noise ratio, defining the optimal DNA probe density, probe-to-lateral spacer ratio (1:0, 1:1, 1:10 and 1:100) and length of a vertical spacer of the probe as well as investigating the effect of different types of lateral spacers.

Enhanced solid-phase recombinase polymerase amplification and electrochemical detection Read More

Low-cost genotyping method based on allele-specific recombinase polymerase amplification and colorimetric microarray detection

By itineris_a2nfh3osnde8e4

An amplification chip platform containing 100 wells was manufactured with a 3D printer and using thermoplastic polylactic acid. The platform reduces reagent consumption and allows parallelization.

Low-cost genotyping method based on allele-specific recombinase polymerase amplification and colorimetric microarray detection Read More

Aboriginal mitogenomes reveal 50,000 years of regionalism in Australia

By itineris_a2nfh3osnde8e4

Here we report 111 mitochondrial genomes (mitogenomes) from historical Aboriginal Australian hair samples, whose origins enable us to reconstruct Australian phylogeographic history before European settlement.

Aboriginal mitogenomes reveal 50,000 years of regionalism in Australia Read More

Instant, Visual, and Instrument-Free Method for On-Site Screening of GTS 40-3-2 Soybean Based on Body-Heat Triggered Recombinase Polymerase Amplification

By itineris_a2nfh3osnde8e4

In this paper, a simple, visual and instrument-free method for instant on-site detection of GTS 40-3-2 soybean has been developed. It is based on body-heat recombinase polymerase amplification (RPA) and followed with naked-eye detection via fluorescent DNA dye.

Instant, Visual, and Instrument-Free Method for On-Site Screening of GTS 40-3-2 Soybean Based on Body-Heat Triggered Recombinase Polymerase Amplification Read More

Self-powered integrated microfluidic point-of-care low-cost enabling (SIMPLE) chip

By itineris_a2nfh3osnde8e4

We report an integrated microfluidic diagnostic device capable of on-site quantitative nucleic acid detection directly from the blood without separate sample preparation steps.

Self-powered integrated microfluidic point-of-care low-cost enabling (SIMPLE) chip Read More

Development of an isothermal recombinase polymerase amplification assay for rapid detection of pseudorabies virus

By itineris_a2nfh3osnde8e4

Recombinase polymerase amplification assays using real-time fluorescent detection (real-time RPA assay) and lateral flow dipstick (RPA LFD assay) were developed targeting the gD gene of pseudorabies virus (PRV).

Development of an isothermal recombinase polymerase amplification assay for rapid detection of pseudorabies virus Read More

Nucleic acid detection with CRISPR-Cas13a/C2c2

By itineris_a2nfh3osnde8e4

We use this Cas13a-based molecular detection platform, termed Specific High-Sensitivity Enzymatic Reporter UnLOCKing (SHERLOCK), to detect specific strains of Zika and Dengue virus, distinguish pathogenic bacteria, genotype human DNA, and identify mutations in cell-free tumour DNA.

Nucleic acid detection with CRISPR-Cas13a/C2c2 Read More

Development of a recombinase polymerase amplification lateral flow dipstick (RPA-LFD) for the field diagnosis of caprine arthritis-encephalitis virus (CAEV) infection

By itineris_a2nfh3osnde8e4

Under the optimal incubation conditions, specifically, 30 min at 37 °C for RPA followed by 5 min at room temperature for LFD, the assay was found to be sensitive to a lower limit of 80 pg of total DNA and 10 copies of plasmid DNA. Furthermore, there was no cross-reaction with other tested viruses, including goat pox virus and bovine

Development of a recombinase polymerase amplification lateral flow dipstick (RPA-LFD) for the field diagnosis of caprine arthritis-encephalitis virus (CAEV) infection Read More

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