PublicationsPost Type
Herein, we established and optimized an LF-RPA method to detect the cytochrome b gene of T. annulata mitochondrial DNA from experimentally infected and field-collected blood samples.
In this study, we evaluate the efficacy of the RPA assay, incubating clinical specimens of HPV16 and HPV18 using plasmids standard. It operates at constant low temperature without the thermal instrumentation for incubation.
In this study, reverse transcription recombinase polymerase amplification assays using real-time fluorescent detection (real-time RT-RPA assay) and lateral flow strip detection (LFS RT-RPA assay) were developed targeting the N gene of PPRV.
A rapid and sensitive AHPND-RPA assay was developed for the specific detection of the AHPND-causing Vibrio owensii. The AHPND-RPA detected as few as 2 copies per reaction in 9.02 ± 0.66 min at 39 °C
• Designed multiple RT-RPA primer sets for Rose rosette virus (RRV). • RT-RPA assay for detection of RRV was developed. • The RPA primer sets were highly specific to RRV. • Primer sets were highly sensitive, detecting up to 1 fg of virus. • Developed assays are rapid, sensitive and reliable. • Developed assays successfully detected RRV from leaves, stems
We present a new approach which enables lysis, extraction, and detection of inactivated Listeria monocytogenes cells from blood using isotachophoresis (ITP) and recombinase polymerase amplification (RPA).
• Non-invasive detection of multiple gene fusion biomarkers in patient urine samples. • 60 min assay which is at least five times faster than traditional multiplexed ligase-based assays. • Requires only 30 ng of starting total RNA sample and has 2 amol detection sensitivity. • Accuracy validated with quantitative real-time PCR. • Good reproducibility at CV=8.9%.
We report on the characterisation of RPA and PCR tests to detect Fasciola infection in clinical stool samples with low egg burdens. The sensitivity of the RPA and PCR were 87% and 66%, respectively. Both tests were 100% specific showing no cross-reactivity with trematode, cestode, or nematode parasites.
In this report, we describe the development of a reverse transcription isothermal recombinase polymerase amplification (RT-RPA) assay for the identification of ZIKV. RT-RPA assay was portable, sensitive (21 RNA molecules), and rapid (3-15 minutes).
The aim of this study was to develop and evaluate a rapid real-time reverse transcription recombinase polymerase amplification (RT-RPA) assay for detection of CV-A6. The sensitivity of this assay was 202 copies/reaction, with 100 % specificity.
