PublicationsPost Type
A novel fluorescent probe-based real-time reverse transcription recombinase polymerase amplification (real-time RT-RPA) assay was developed for rapid detection of highly pathogenic type 2 porcine reproductive and respiratory syndrome virus (HP-PRRSV).
•Development of a multiplex real-time RPA assay to detect Campylobacter coli & jejuni. •Quantitative detection within a 20 min runtime using the multiplex Rti-RPA assay. •Equivalent or better detection sensitivity than other PCR/LAMP assays. •Fast procedure, fairly simple, and specific machinery not required. •Applicable in the surveillance of Campylobacter contamination in poultry products.
An innovative method termed aptamer-recombinase polymerase amplification (Apta-RPA) exploiting the affinity and specificity of a DNA aptamer selected against the anaphylactic β-conglutin allergen termed β-conglutin binding aptamer II (β-CBA II), facilitating ultrasensitive detection via isothermal amplification.
Development of Quantitative Real-time Recombinase Polymerase Amplification (qRT-RPA) Method for Quantitative Detection against Pathogenic Virus in Honeybee
꿀벌 병원성 바이러스의 정량적 검출을 위한 정량 실시간 재조합효소-중합효소 증폭법의 개발 Read More
The aim of this study was to develop and evaluate a rapid real-time reverse transcription recombinase polymerase amplification (RT-RPA) assay for detection of CV-A6. The sensitivity of this assay was 202 copies/reaction, with 100 % specificity.
We report on the characterisation of RPA and PCR tests to detect Fasciola infection in clinical stool samples with low egg burdens. The sensitivity of the RPA and PCR were 87% and 66%, respectively. Both tests were 100% specific showing no cross-reactivity with trematode, cestode, or nematode parasites.
• Non-invasive detection of multiple gene fusion biomarkers in patient urine samples. • 60 min assay which is at least five times faster than traditional multiplexed ligase-based assays. • Requires only 30 ng of starting total RNA sample and has 2 amol detection sensitivity. • Accuracy validated with quantitative real-time PCR. • Good reproducibility at CV=8.9%.
Here we report on the development of a point-of-care nucleic acid lateral flow test for the direct detection of isothermally amplified DNA. The recombinase polymerase amplification method is modified slightly to use tailed primers, resulting in an amplicon with a duplex flanked by two single stranded DNA tails.
We present a new approach which enables lysis, extraction, and detection of inactivated Listeria monocytogenes cells from blood using isotachophoresis (ITP) and recombinase polymerase amplification (RPA).
• Designed multiple RT-RPA primer sets for Rose rosette virus (RRV). • RT-RPA assay for detection of RRV was developed. • The RPA primer sets were highly specific to RRV. • Primer sets were highly sensitive, detecting up to 1 fg of virus. • Developed assays are rapid, sensitive and reliable. • Developed assays successfully detected RRV from leaves, stems
