PublicationsPost Type
The aim of this study was to develop and evaluate a rapid real-time reverse transcription recombinase polymerase amplification (RT-RPA) assay for detection of CV-A6. The sensitivity of this assay was 202 copies/reaction, with 100 % specificity.
Development of Quantitative Real-time Recombinase Polymerase Amplification (qRT-RPA) Method for Quantitative Detection against Pathogenic Virus in Honeybee
꿀벌 병원성 바이러스의 정량적 검출을 위한 정량 실시간 재조합효소-중합효소 증폭법의 개발 Read More
An innovative method termed aptamer-recombinase polymerase amplification (Apta-RPA) exploiting the affinity and specificity of a DNA aptamer selected against the anaphylactic β-conglutin allergen termed β-conglutin binding aptamer II (β-CBA II), facilitating ultrasensitive detection via isothermal amplification.
•Development of a multiplex real-time RPA assay to detect Campylobacter coli & jejuni. •Quantitative detection within a 20 min runtime using the multiplex Rti-RPA assay. •Equivalent or better detection sensitivity than other PCR/LAMP assays. •Fast procedure, fairly simple, and specific machinery not required. •Applicable in the surveillance of Campylobacter contamination in poultry products.
•A low cost, optical, POC molecular diagnostic platform. •Sample preparation using a paper membrane. •Isothermal amplification using HDA and RPA.
Modular development of a prototype POC molecular diagnostic platform for STIs Read More
A highly sensitive and specific RPA-based detection system for Genuity Roundup Ready 2 Yield (RR2Y) material in soybean (Glycine max) seed samples applicable in both laboratory and field-type settings.
Electrochemical detection of solid-phase RPA of the salmon pathogen Piscirickettsia salmonis in salmon genomic DNA. The system was applied to the analysis of eight real salmon samples, and the method was also compared to qPCR analysis, observing an excellent degree of correlation.
Electrochemical detection of Piscirickettsia salmonis genomic DNA from salmon samples Read More
This study describes a novel EGFR mutation detection technique. Compared to direct DNA sequencing detection methods, this method is based on allele-specific amplification (ASA), recombinase polymerase amplification (RPA), peptide nucleic acid (PNA), and SYBR Green I (SYBR), referred to as the AS-RPA-PNA-SYBR (ARPS) system.
A Novel Technique to Detect EGFR Mutations in Lung Cancer Read More
An RPA test for Leishmania donovani was successfully tested using a suitcase laboratory in a hospital in Bangladesh. Forty-eight positive and 48 negative samples showed 100% correlation with PCR results.
Mobile suitcase laboratory for rapid detection of Leishmania donovani using RPA assay Read More
An RPA test that detects 0.5 oocyst per reaction, with no cross reactivity to other common protozoan species in the intestine of dairy cattle. A simple, rapid, and cost-effective test, with the potential for further development as a diagnostic kit for the diagnosis of cryptosporidiosis of dairy cattle.
