PublicationsPost Type
A new approach to capture, concentrate and prepare amplification-ready DNA from antibiotic resistant bacteria in human urine samples. Bacteria were captured using anion exchange diaethylaminoethyl (DEAE) magnetic microparticles and concentrated 200-fold within ~3.5 min using a custom, valve-less microfluidic chip.
dRPA-on-chip processing required less than 30 min, which was a 4-fold decrease compared to dPCR, requiring approximately 2 h. dRPA on the PWA chip provides a simple and highly sensitive method to quantify nucleic acids without thermal cycling or precise micropump/microvalve control.
Picoliter Well Array Chip-Based Digital RPA for Absolute Quantification of Nucleic Acids Read More
A novel molecular method (RPA-LF) that could be applied in the field because it does not require sophisticated equipment. It is also very sensitive and specific to detect the principal Leishmania species that produce cutaneous leishmaniasis in Latin America.
Recombinase Polymerase Amplification (RPA), combines this advantage of isothermal PCR with simplicity and rapid amplification. A mathematical model is presented of Recombinase Polymerase Amplification (RPA) and compared to experimental data.
•A low cost, optical, POC molecular diagnostic platform. •Sample preparation using a paper membrane. •Isothermal amplification using HDA and RPA.
Modular development of a prototype POC molecular diagnostic platform for STIs Read More
A highly sensitive and specific RPA-based detection system for Genuity Roundup Ready 2 Yield (RR2Y) material in soybean (Glycine max) seed samples applicable in both laboratory and field-type settings.
Electrochemical detection of solid-phase RPA of the salmon pathogen Piscirickettsia salmonis in salmon genomic DNA. The system was applied to the analysis of eight real salmon samples, and the method was also compared to qPCR analysis, observing an excellent degree of correlation.
Electrochemical detection of Piscirickettsia salmonis genomic DNA from salmon samples Read More
This study describes a novel EGFR mutation detection technique. Compared to direct DNA sequencing detection methods, this method is based on allele-specific amplification (ASA), recombinase polymerase amplification (RPA), peptide nucleic acid (PNA), and SYBR Green I (SYBR), referred to as the AS-RPA-PNA-SYBR (ARPS) system.
A Novel Technique to Detect EGFR Mutations in Lung Cancer Read More
An RPA test for Leishmania donovani was successfully tested using a suitcase laboratory in a hospital in Bangladesh. Forty-eight positive and 48 negative samples showed 100% correlation with PCR results.
Mobile suitcase laboratory for rapid detection of Leishmania donovani using RPA assay Read More
An RPA test that detects 0.5 oocyst per reaction, with no cross reactivity to other common protozoan species in the intestine of dairy cattle. A simple, rapid, and cost-effective test, with the potential for further development as a diagnostic kit for the diagnosis of cryptosporidiosis of dairy cattle.
