PublicationsPost Type
dRPA-on-chip processing required less than 30 min, which was a 4-fold decrease compared to dPCR, requiring approximately 2 h. dRPA on the PWA chip provides a simple and highly sensitive method to quantify nucleic acids without thermal cycling or precise micropump/microvalve control.
Picoliter Well Array Chip-Based Digital RPA for Absolute Quantification of Nucleic Acids Read More
Recombinase Polymerase Amplification (RPA), combines this advantage of isothermal PCR with simplicity and rapid amplification. A mathematical model is presented of Recombinase Polymerase Amplification (RPA) and compared to experimental data.
•A low cost, optical, POC molecular diagnostic platform. •Sample preparation using a paper membrane. •Isothermal amplification using HDA and RPA.
Modular development of a prototype POC molecular diagnostic platform for STIs Read More
A highly sensitive and specific RPA-based detection system for Genuity Roundup Ready 2 Yield (RR2Y) material in soybean (Glycine max) seed samples applicable in both laboratory and field-type settings.
For the first time we demonstrate a self-sufficient lab-on-a-foil system for the fully automated analysis of nucleic acids which is based on the recently available isothermal recombinase polymerase amplification (RPA).
In this paper, digital quantitative detection of nucleic acids was achieved at the single-molecule level by chemical initiation of over one thousand sequence-specific, nanoliter isothermal amplification reactions in parallel.
The one-step-RT-RPA assay showed a sensitivity of 19 molecules detected as determined by probit analysis of eight runs using a RVFV S-segment based quantitative RNA standard and detected 20 different RVFV strains.
Recombinase polymerase amplification assay for rapid detection of Rift Valley fever virus Read More
The RPA-ELISA combination is proposed for amplification at a low, constant temperature (40°C) in a short time (40min), for the hybridisation of labelled products to specific 5′-biotinylated probes/streptavidin in coated microtiter plates at room temperature, and for detection by colorimetric immunoassay.
Immunosorbent assay as a DNA amplification-detection strategy for food analysis Read More
We demonstrate the potential of isothermal recombinase polymerase amplification assay as a clinically useful molecular diagnostic tool that is simple and faster than PCR/RT-PCR. Recombinase polymerase amplification offers great potential for nucleic acid-based diagnostics at the point of care.
RPA detection of Cryptosporidium species in DNA extracted from stool samples. TwistAmp nfo based assays were more sensitive than RT-PCR and only needed 30 minutes of amplification time. Using a simple paper and plastic device, lateral flow strips were one to two orders of magnitude more sensitive than detection of products by gel electrophoresis.
