PublicationsPost Type
RPA detection of Cryptosporidium species in DNA extracted from stool samples. TwistAmp nfo based assays were more sensitive than RT-PCR and only needed 30 minutes of amplification time. Using a simple paper and plastic device, lateral flow strips were one to two orders of magnitude more sensitive than detection of products by gel electrophoresis.
Based on a recently reported ultra-sensitive bio-barcode (BBC) assay, we have developed an aptamer-based bio-barcode (ABC) alternative to detect a cell death marker cytochrome-c (Cyto-c) and its subsequent application to screen anti-cancer drugs.
Aptamer-based Bio-barcode Assay for Cytochrome-c Detection Read More
Recombinase polymerase amplification (RPA) is a novel technology that is ideal for an HIV-1 diagnostic, as it amplifies target DNA in
Rapid Detection of HIV-1 Proviral DNA Read More
Syndromic panels for infectious disease have been suggested to be of value in point-of-care diagnostics for developing countries and for biodefense. To test the performance of isothermal recombinase polymerase amplification (RPA) assays, we developed a panel of 10 RPAs for biothreat agents.
Development of a panel of RPA assays for the detection of biothreat agents Read More
With a view to developing a rapid pathogen detection system utilizing isothermal nucleic acid amplification, the necessary micro-mixing step is innovatively implemented on a chip. Passive laminar flow mixing of two 6.5 μl batches differing in viscosity is performed within a microfluidic chamber.
A phaseguided passive batch microfluidic mixing chamber for isothermal amplification Read More
We have developed a device constructed of layers of paper, glass fiber, and plastic that is capable of performing isothermal, enzymatic amplification of HIV DNA.Â
A paper & plastic device to perform recombinase polymerase amplification of HIV DNA Read More
Oyola et al compared a number of different polymerases and PCR methods with RPA for generating libraries for sequencing AT-rich genomes. The TwistAmp Basic reactions were used without optimisation, simply following the instructions in the Quick Guide.
Optimizing Illumina next-gen sequencing library preparation for at-biased genomes Read More
We report the development of a novel qualitative real-time isothermal recombinase polymerase amplification (RPA) assay for use on a small ESEQuant Tube Scanner device. The analytical sensitivity and specificity were tested using a plasmid standard and DNA extracts from infected rabbit tissues.
Recombinase Polymerase amplification assay for rapid detection of Francisella tularensis Read More
In this paper, digital quantitative detection of nucleic acids was achieved at the single-molecule level by chemical initiation of over one thousand sequence-specific, nanoliter isothermal amplification reactions in parallel.
For the first time we demonstrate a self-sufficient lab-on-a-foil system for the fully automated analysis of nucleic acids which is based on the recently available isothermal recombinase polymerase amplification (RPA).
