PublicationsPost Type
Oyola et al compared a number of different polymerases and PCR methods with RPA for generating libraries for sequencing AT-rich genomes. The TwistAmp Basic reactions were used without optimisation, simply following the instructions in the Quick Guide.
Optimizing Illumina next-gen sequencing library preparation for at-biased genomes Read More
Recombinase polymerase amplification (RPA) is a novel technology that is ideal for an HIV-1 diagnostic, as it amplifies target DNA in
Rapid Detection of HIV-1 Proviral DNA Read More
Syndromic panels for infectious disease have been suggested to be of value in point-of-care diagnostics for developing countries and for biodefense. To test the performance of isothermal recombinase polymerase amplification (RPA) assays, we developed a panel of 10 RPAs for biothreat agents.
Development of a panel of RPA assays for the detection of biothreat agents Read More
With a view to developing a rapid pathogen detection system utilizing isothermal nucleic acid amplification, the necessary micro-mixing step is innovatively implemented on a chip. Passive laminar flow mixing of two 6.5 μl batches differing in viscosity is performed within a microfluidic chamber.
A phaseguided passive batch microfluidic mixing chamber for isothermal amplification Read More
Solid-phase RPA to simultaneously amplify and detect Neisseria gonorrhoeae, Salmonella enterica, methicillin-resistant Staphylococcus aureus (MRSA) and a control plasmid in
Multiplex isothermal solid-phase RPA for DNA-based detection of three bacterial pathogens Read More
RPA assays were developed to detect Yellow Fever Virus on three different platforms (real-time with or without microfluidic semi-automated system and lateral-flow assay). The assay was able to detect 20 different YFV strains with no cross-reactions with closely related viruses. The RPA assay proved to be a robust, portable method with a low detection limit (
Rapid Molecular Assays for the Detection of Yellow Fever Virus in Low-Resource Settings Read More
TwistFlow® Salmonella tests integrated into a centrifugal microfluidic device that performs DNA extraction, amplification and detection onto a single disc. DNA extraction to readout took 30 minutes with sensitivities of 10 CFU/ml of PBS and 100 CFU/ml of milk.
A Fully Integrated Lab-on-a-Disc for Nucleic Acid Analysis of Food-borne Pathogens Read More
Among a wide range of available technologies, high-speed real-time reverse transcriptase quantitative PCR (RT-qPCR) and the two isothermal amplification techniques loop-mediated isothermal amplification (LAMP) and recombinase polymerase amplification (RPA) represent three promising candidates for integration into mobile pen-side tests.
First demonstration of the coupling of digital plasma separation with RPA nucleic acid detection directly from blood samples.
One-step digital plasma separation for molecular diagnostics Read More
RPA assays developed to detect Shiga toxin-producing Escherichia coli (STEC), a major family of foodborne pathogens of public health, zoonotic, and economic significance in the United States and worldwide.
Real-Time Isothermal Detection of Shiga Toxin-Producing Escherichia coli Using RPA Read More
