PublicationsPost Type
Recombinase Polymerase Amplification combined with a lateral flow dipstick for discriminating between infectious Penaeus stylirostris densovirus and virus-related sequences in shrimp genome.
This paper describes the use of an internal control and an algorithm to quantify HIV-1 DNA using TwistAmp exo reactions.
Quantification of HIV-1 DNA using Real-Time Recombinase Polymerase Amplification Read More
Leptospirosis is a difficult to disease to diagnose. Researchers from KIT Biomedical Research have developed an RPA test that can detect
Development of a RPA Assay for the Detection of Pathogenic Leptospira Read More
RT-RPA and lateral flow used to detect the Little cherry virus 2 coat protein gene. RT-RPA was simple, fast, and specific. The test has the sensitivity of RT-PCR and is more cost effective, being capable of detecting the virus from crude extracts from field samples.
RPA assays developed to detect Shiga toxin-producing Escherichia coli (STEC), a major family of foodborne pathogens of public health, zoonotic, and economic significance in the United States and worldwide.
Real-Time Isothermal Detection of Shiga Toxin-Producing Escherichia coli Using RPA Read More
First demonstration of the coupling of digital plasma separation with RPA nucleic acid detection directly from blood samples.
One-step digital plasma separation for molecular diagnostics Read More
Among a wide range of available technologies, high-speed real-time reverse transcriptase quantitative PCR (RT-qPCR) and the two isothermal amplification techniques loop-mediated isothermal amplification (LAMP) and recombinase polymerase amplification (RPA) represent three promising candidates for integration into mobile pen-side tests.
TwistFlow® Salmonella tests integrated into a centrifugal microfluidic device that performs DNA extraction, amplification and detection onto a single disc. DNA extraction to readout took 30 minutes with sensitivities of 10 CFU/ml of PBS and 100 CFU/ml of milk.
A Fully Integrated Lab-on-a-Disc for Nucleic Acid Analysis of Food-borne Pathogens Read More
A TwistAmp nfo-based test for the 18S rRNA gene of P. falciparum detected as few as four parasites after
RPA assays were developed to detect Yellow Fever Virus on three different platforms (real-time with or without microfluidic semi-automated system and lateral-flow assay). The assay was able to detect 20 different YFV strains with no cross-reactions with closely related viruses. The RPA assay proved to be a robust, portable method with a low detection limit (
Rapid Molecular Assays for the Detection of Yellow Fever Virus in Low-Resource Settings Read More
