PublicationsPost Type
This paper describes the use of an internal control and an algorithm to quantify HIV-1 DNA using TwistAmp exo reactions.
Quantification of HIV-1 DNA using Real-Time Recombinase Polymerase Amplification Read More
RT-RPA and lateral flow used to detect the Little cherry virus 2 coat protein gene. RT-RPA was simple, fast, and specific. The test has the sensitivity of RT-PCR and is more cost effective, being capable of detecting the virus from crude extracts from field samples.
RPA assays developed to detect Shiga toxin-producing Escherichia coli (STEC), a major family of foodborne pathogens of public health, zoonotic, and economic significance in the United States and worldwide.
Real-Time Isothermal Detection of Shiga Toxin-Producing Escherichia coli Using RPA Read More
First demonstration of the coupling of digital plasma separation with RPA nucleic acid detection directly from blood samples.
One-step digital plasma separation for molecular diagnostics Read More
RPA is combined with quantum dot barcodes to allow multiplex detection of pathogens with a smartphone. RPA boosted the sensitivity of pathogen detection in a situation where PCR was not possible.
The solid-phase amplification assay, including the washing protocols and development reaction, was performed by the dispensation of solutions through the inlet and by controlling the flow-movement by DVD drive centrifugation.
Solid-phase RPA on microfluidic DVDs Read More
The centrifugal microfluidic droplet generation was used to perform the first digital droplet recombinase polymerase amplification (ddRPA). It was used for absolute quantification of Listerias monocytogenes DNA concentration standards with a total analysis time below 30 min – about a quarter of the time it would take for ddPCR.
“Development of a robust and automated solid-phase amplification platform for optical detection of dsDNA targets. Real-time and label-free monitoring of isothermal solid-phase amplification was achieved using ring resonators in combination with recombinase polymerase amplification. The amplification of a 144-bp target was successfully demonstrated with a limit of detection of 7.8-10−13 M (6-105 copies in 50 -L).”
Real-time RT-PCR analysis is not suitable for on-site screening since mobile devices are large, expensive, and complex. In this study, two RT-recombinase polymerase amplification (RT-RPA) assays were developed to detect DENV1-4.
Recombinase Polymerase Amplification Assay for Rapid Diagnostics of Dengue Infection Read More
Yam mosaic virus (YMV; genus Potyvirus) is considered to cause the most economically important viral disease of yams (Dioscorea spp.) in West Africa which is the dominant region for yam production globally… The RT-exoRPA assay has, however, several advantages over the RT-PCR; positive samples can be detected in less than 30 min, and amplification only requires a single incubation temperature (optimum 37 °C).
Rapid and specific detection of Yam mosaic virus by reverse-transcription RPA Read More
