PublicationsPost Type
In this paper, the complete procedures to set up an RPA assay, applied to detection of RNA (Potato virus Y, Potyvirus) and DNA (Wheat dwarf virus, Mastrevirus) viruses, are described. The proposed procedure allows developing species- or subspecies-specific detection assay.
Detection and Characterization of Viral Species/Subspecies Using Isothermal RPA Assays. Read More
A video protocol for developing a real-time recombinase polymerase amplification assay to quantify initial concentration of DNA samples using either a thermal cycler or a microscope and stage heater. Also described is the development of an internal positive control.
Development of a Quantitative RPA Assay with an Internal Positive Control Read More
The solid-phase amplification assay, including the washing protocols and development reaction, was performed by the dispensation of solutions through the inlet and by controlling the flow-movement by DVD drive centrifugation.
Solid-phase RPA on microfluidic DVDs Read More
RPA is combined with quantum dot barcodes to allow multiplex detection of pathogens with a smartphone. RPA boosted the sensitivity of pathogen detection in a situation where PCR was not possible.
RPA (Recombinase Polymerase Amplification) combined with a novel bridging flocculation assay for qualitative evaluation of isothermally amplified plant and other pathogen DNA and RNA by naked eye.
The RPA assay detected as few as 10 copies of DENV RNA in
Early Detection of the Dengue Virus Using RT-RPA Read More
An RPA assay was characterized on the bench top using stool samples spiked with Giardia cysts where it showed a limit-of-detection nearly as low as the gold standard polymerase chain reaction assay. Testing with the assay in the highlands of Peru on 104 stool samples collected from the surrounding communities suggested that further improvements in clinical sensitivity will be needed
Recombinase Polymerase Amplification-Based Assay to Diagnose Giardia in Stool Samples Read More
A brief investigation of the impact of location and number of template/oligonucleotide mismatches on RPA based on assays developed for tuf gene sequence of Pseudomonas aeruginosa and/or Mycobacterium tuberculosis and for the cfb gene sequence of Streptococcus agalactiae.
Influence of sequence mismatches on the specificity of RPA technology Read More
The proposed approaches were successfully compared to conventional PCR and tested for the milk sample analysis as a microarray format on a compact disc (support and driver). Satisfactory results were obtained in terms of resistance to inhibition, selectivity, sensitivity (10(1)-10(2)CFU/mL) and reproducibility (below 12.5%).
Isothermal DNA amplification strategies for duplex microorganism detection. Read More
In this study, we demonstrated that human body heat may be harnessed to incubate recombinase polymerase amplification (RPA) reactions for isothermal amplification of HIV-1 DNA.
