Publications

PublicationsPost Type

High-specificity Double-stranded DNA Detection with “Humanoid” Molecular Beacon and TALEs.

By

Owing to the limitation of DNA-specific recognition, improving the sensitivity of bacteria detection without additional culture poses a considerable challenge. Herein, a sensitive, novel, and high-specificity fluorescence detection method was developed using transcription activator-like effectors (TALEs) as the specifically recognized element. Based on the property of TALEs for recognizing double-stranded DNA, the detection method was first proposed. Its sensitivity was […]

High-specificity Double-stranded DNA Detection with “Humanoid” Molecular Beacon and TALEs. Read More

Reverse transcription-recombinase polymerase amplification combined with lateral flow strip for detection of rice black-streaked dwarf virus in plants

By

Rice black-streaked dwarf virus (RBSDV) infects rice plants, a major crop, and is transmitted via the small brown planthopper (SBPH: Laodelphax striatellus Fallén), causing significant economic loss in China. To rapidly diagnose RBSDV, a reverse transcription-recombinase polymerase amplification (RT-RPA) method was developed using P10 virus-specific primers and probes.

Reverse transcription-recombinase polymerase amplification combined with lateral flow strip for detection of rice black-streaked dwarf virus in plants Read More

Duplex lateral flow assay for the simultaneous detection of Yersinia pestis and Francisella tularensis.

By

The multiplexed amplification detection of genomic DNA from Francisella tularensis and Yersinia pestis is reported here. Amplification was achieved using isothermal recombinase polymerase amplification (RPA), exploiting tailed primers, followed by detection using a nucleic-acid lateral flow assay.

Duplex lateral flow assay for the simultaneous detection of Yersinia pestis and Francisella tularensis. Read More

Genome-informed diagnostics for specific and rapid detection of Pectobacterium species using recombinase polymerase amplification coupled with a lateral flow device.

By

Recombinase polymerase amplification (RPA) combined with a lateral flow device (LFD) was developed for specific detection of Pectobacterium sp. directly from infected plant materials with no need for DNA isolation. The specificity of RPA-LFD was tested with 26 Pectobacterium sp. strains and 12 non-Pectobacterium species and no false positive or false negative outcomes were observed. RPA primers and probe for

Genome-informed diagnostics for specific and rapid detection of Pectobacterium species using recombinase polymerase amplification coupled with a lateral flow device. Read More

Development of Molecular Methods to Detect Macrophomina phaseolina from Strawberry Plants and Soil.

By

RPA has been validated on over 200 infected strawberry plants with a diagnostic sensitivity of 93% and a diagnostic specificity of 99%, respectively. Together, this work demonstrates the value of using new approaches to identify loci for detection and provides valuable diagnostic tools that can be used to monitor soil and strawberry plant samples for M. phaseolina.

Development of Molecular Methods to Detect Macrophomina phaseolina from Strawberry Plants and Soil. Read More

A lateral flow dipstick combined with reverse transcription recombinase polymerase amplification for rapid and visual detection of the bovine respirovirus 3.

By

In this study, a novel isothermal assay for detecting BPIV3 was established. The method includes a lateral flow dipstick (LFD) assay combined with reverse transcription recombinase polymerase amplification (RT-RPA).

A lateral flow dipstick combined with reverse transcription recombinase polymerase amplification for rapid and visual detection of the bovine respirovirus 3. Read More

Naked eye detection of the Mycobacterium tuberculosis complex by recombinase polymerase amplification-SYBR green I assays.

By

Rapid diagnosis of Mycobacterium tuberculosis (Mtb) is key to controlling the spread of tuberculosis, which is a global health concern. In this study, isothermal recombinase polymerase amplification (RPA) was developed to detect specific targets of Mtb, IS6110 and IS1081. Additionally, SYBR Green I was used for endpoint detection of the RPA products by the naked eye.

Naked eye detection of the Mycobacterium tuberculosis complex by recombinase polymerase amplification-SYBR green I assays. Read More

Detection and quantification of the toxic marine microalgae Karlodinium veneficum and Karlodinium armiger using recombinase polymerase amplification and enzyme-linked oligonucleotide assay.

By

An assay for the detection of two Karlodinium species has been developed. The assay is based on isothermal DNA amplification and a sandwich hybridisation. The assay discriminates between Karlodinium veneficum and Karlodinium armiger. Seawater samples spiked with Karlodinium cells have been analysed. Excellent correlations between the assay and light microscopy have been obtained.

Detection and quantification of the toxic marine microalgae Karlodinium veneficum and Karlodinium armiger using recombinase polymerase amplification and enzyme-linked oligonucleotide assay. Read More

Shopping Basket

Start typing and press enter to search