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Detection of root-infecting fungi on cool-season turfgrasses using loop-mediated isothermal amplification and recombinase polymerase amplification.

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Take-all patch, necrotic ring spot, and summer patch are destructive diseases of amenity turfgrasses in temperate climates. LAMP and RPA assays were created for each of the causal agents of the above diseases. Both assays were highly specific to each pathogen and consistently amplified the target organism. RPA assays in general demonstrated a lower tendency for false positive results in […]

Detection of root-infecting fungi on cool-season turfgrasses using loop-mediated isothermal amplification and recombinase polymerase amplification. Read More

Rapid and visual detection of Group B streptococcus using recombinase polymerase amplification combined with lateral flow strips.

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A rapid molecular method for GBS detection was developed and evaluated. This assay has high specificity and sensitivity for diagnosing GBS, which is suitable for clinical application. The assay can be completed within about 30 minutes without the need of sophisticated instruments. This is a suitable method for use in point-of-care test.

Rapid and visual detection of Group B streptococcus using recombinase polymerase amplification combined with lateral flow strips. Read More

Diagnostic accuracy of a prototype rapid chlamydia and gonorrhoea recombinase polymerase amplification assay: a multi-centre cross-sectional pre-clinical evaluation.

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This prototype test has excellent performance characteristics, comparable to currently used NAATs, and fulfils several World Health Organization ASSURED criteria. Its rapidity without loss of performance suggests that once further developed and commercialized, this test could positively affect clinical practice and public health.

Diagnostic accuracy of a prototype rapid chlamydia and gonorrhoea recombinase polymerase amplification assay: a multi-centre cross-sectional pre-clinical evaluation. Read More

A novel recombinase polymerase amplification (RPA) assay for the rapid isothermal detection of Neospora caninum in aborted bovine fetuses

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A recombinase polymerase amplification (RPA) assay combined with lateral flow (LF) strips was developed for the detection of N. caninum. The LF-RPA assay distinguished N. caninum from nine closely related protozoan species. The amplification reaction was completed in about 10 min under isothermal condition. The LF-RPA assay can detect N. caninum DNA at amounts as low as 50 fg.

A novel recombinase polymerase amplification (RPA) assay for the rapid isothermal detection of Neospora caninum in aborted bovine fetuses Read More

Development of a rapid and visual nucleotide detection method towards an Chinese epidemic strain of Orientia tsutsugamushi based on recombinase polymerase amplification assay and lateral flow test

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The RPA–LF method could differentiate O. tsutsugamushi from other phylogenetically related bacteria. The sensitivity was 100% and specificity was over 90%, when evaluated using infected animal samples or simulative clinical samples. Furthermore, the method was completed in 20 min at 37 °C followed by a 3–5 min incubation at room temperature for the development of an immunochromatographic strip, and the

Development of a rapid and visual nucleotide detection method towards an Chinese epidemic strain of Orientia tsutsugamushi based on recombinase polymerase amplification assay and lateral flow test Read More

Recombinase Polymerase Amplification Combined with Lateral Flow Strip for Listeria monocytogenes Detection in Food.

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Experiments confirmed a detection limit as low as 300 fg of DNA and 1.5 × 101 CFU in pure cultures. Furthermore, RPA‐LF exhibited no cross‐reactions with pathogens. Evaluation of the method with food samples indicated that the detection limit was substantially improved to 1.5 × 10° CFU for the original bacterial content in 25 g/mL samples after enrichment for 6

Recombinase Polymerase Amplification Combined with Lateral Flow Strip for Listeria monocytogenes Detection in Food. Read More

Development of a Reverse Transcription-Recombinase Polymerase Amplification Assay for Detection of Sugarcane Yellow Leaf Virus

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The RT-RPA assay showed the same results as those of RT-PCR assay, indicating that the former was highly reliable for SCYLV detection. Analysis of the temperature and time limits revealed a wide operating temperature range from 27 to 45 °C, which was easily reached, and a rapid assay duration of 20 min.

Development of a Reverse Transcription-Recombinase Polymerase Amplification Assay for Detection of Sugarcane Yellow Leaf Virus Read More

Rapid detection of transmissible gastroenteritis virus in swine small intestine samples using real-time reverse transcription recombinase polymerase amplification

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The assay performance was evaluated by testing 76 clinical samples by RT-RPA and a real-time RT-PCR. Fourteen samples were TGEV RNA positive in RT-RPA (18.4%, 14/76), which were also positive in the real-time RT-PCR. The diagnostic agreement between the two assays was 100% (76/76).

Rapid detection of transmissible gastroenteritis virus in swine small intestine samples using real-time reverse transcription recombinase polymerase amplification Read More

Naked-eye and electrochemical detection of isothermally amplified HOTAIR long non-coding RNA

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Herein, we report on the development of a new colorimetric and electrochemical assay platform for long non-coding HOX transcript antisense intergenic RNA (HOTAIR) detection. Isothermal reverse transcription-recombinase polymerase amplification (RT-RPA) was performed to amplify HOTAIR sequences from a RNA pool extracted from a designated number of ovarian cancer cells and a small cohort of plasma samples derived from patients with

Naked-eye and electrochemical detection of isothermally amplified HOTAIR long non-coding RNA Read More

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